Comparison of insecticide toxicity research results performed using induct-test and microcosm<\/p>\n
1Josip Milas, 2Branimir K- Hackenberger, 2Davorka Jari\u0107, 2Enrih Merdi\u0107<\/strong> Abstract: Comparison of insecticide toxicity research results performed using induct-test and microcosm<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[9],"tags":[50,33,47,46,48,51,49],"class_list":["post-139","post","type-post","status-publish","format-standard","hentry","category-11_th_march","tag-carbofuran","tag-croatia","tag-induct-test","tag-insecticide","tag-microcosm","tag-oxidative-stress","tag-temephos"],"_links":{"self":[{"href":"https:\/\/www.openstream.tv\/zanzare\/wp-json\/wp\/v2\/posts\/139","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.openstream.tv\/zanzare\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.openstream.tv\/zanzare\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.openstream.tv\/zanzare\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.openstream.tv\/zanzare\/wp-json\/wp\/v2\/comments?post=139"}],"version-history":[{"count":3,"href":"https:\/\/www.openstream.tv\/zanzare\/wp-json\/wp\/v2\/posts\/139\/revisions"}],"predecessor-version":[{"id":166,"href":"https:\/\/www.openstream.tv\/zanzare\/wp-json\/wp\/v2\/posts\/139\/revisions\/166"}],"wp:attachment":[{"href":"https:\/\/www.openstream.tv\/zanzare\/wp-json\/wp\/v2\/media?parent=139"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.openstream.tv\/zanzare\/wp-json\/wp\/v2\/categories?post=139"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.openstream.tv\/zanzare\/wp-json\/wp\/v2\/tags?post=139"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
\n1Public Health Institute of the Osijek-Baranja County, Osijek, Croatia<\/strong>
\n2Josip Juraj Strossmayer University, Department of Biology, Osijek, Croatia<\/strong><\/p>\n
\nInduct-test is a standard procedure for testing toxicity of various chemicals for experimental organisms after intraperitoneal (i.p.) administration of tested compound and \u201cincubation\u201d period of 4-12 days, while a microcosm as a procedure for toxicity testing
\nrelies on imitating natural conditions and routes of exposure. Tested insecticides were malathion, temephos and carbofuran. Experimental organisms used were common carp (Cyprinus carpio) in both experiments and, additionally, prussian carp (Carrasius
\nauratus gibelio) in the induct-test.
\nMeasured enzyme biomarkers in both experiments were: Ethoxyresorufin-O-deethylase (EROD), Total Glutathione (GT), Glutathione-S-transferase (GST), Thiobarbituric Acid
\nReactive Substances (TBARS), Acetylcholinesterase activity (AChE) and Catalase activity (CAT). The microcosms contained 150 L of water, 35 specimens of common carp, artificial soil as sediment and 10 specimens of swan mussel (Anodonta cygnea) as a
\nfilter-feeding organism. The pollutants were administered in realistically expected concentrations and 7 fish were sampled every 5 days, with a total of 5 samplings.
\nThe results showed expected differences in biomarker change between induct-test and microcosm experiment (40- to 100-fold). The concentrations used in the induct-test are hardly expected in the environment even if species bioaccumulation potential is taken
\ninto account. On the other hand, the microcosm experiment showed that all biomarkers used in the experiment can detect changes obtained by environmentally realistic concentrations and, therefore, microcosm test has a far more realistic and significant role
\nin ecological risk assessment of insecticides.<\/p>\n","protected":false},"excerpt":{"rendered":"